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Related Concept Videos

The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview

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Related Experiment Video

Updated: Jul 9, 2026

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
06:25

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

Published on: February 10, 2023

DNA replication stress, genome instability and aging.

William C Burhans1, Martin Weinberger

  • 1Department of Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA. wburhans@buffalo.edu

Nucleic Acids Research
|December 7, 2007
PubMed
Summary

Genome instability, a key aging factor, may stem from DNA replication stress, not just oxidative damage. This review explores how growth signaling and reactive oxygen species (ROS) contribute to replication stress and aging.

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Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model
08:46

Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model

Published on: September 29, 2011

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Last Updated: Jul 9, 2026

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
06:25

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

Published on: February 10, 2023

Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model
08:46

Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model

Published on: September 29, 2011

Area of Science:

  • Gerontology
  • Molecular Biology
  • Genetics

Background:

  • Genome instability is a critical aspect of aging across eukaryotes, but its origins are not fully understood.
  • The free radical theory of aging suggests oxidative DNA damage from mitochondria-derived reactive oxygen species (ROS) is a primary cause, though evidence is mixed.
  • Enhanced growth signaling is also implicated in aging, with recent research highlighting its role in inducing DNA replication stress.

Purpose of the Study:

  • To review evidence linking DNA replication stress and subsequent genome instability to the aging process.
  • To explore the interplay between growth signaling, reactive oxygen species (ROS), and DNA replication stress in aging.

Main Methods:

  • Literature review of studies on aging, genome instability, oxidative stress, and DNA replication stress.
  • Analysis of experimental evidence from model organisms like Saccharomyces cerevisiae and higher eukaryotes.

Main Results:

  • Evidence suggests that DNA replication stress, induced by growth signaling and potentially ROS, leads to DNA damage and genome instability.
  • Replication stress has been an underappreciated factor contributing to age-related genome instability and diseases.

Conclusions:

  • DNA replication stress and the resulting genome instability are significant, previously overlooked, factors in aging.
  • Understanding these mechanisms could offer new insights into age-related diseases such as cancer and neurodegeneration.