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Related Experiment Video

Updated: Jul 9, 2026

Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence
09:18

Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence

Published on: January 29, 2019

Quantitative analysis of synaptic phosphorylation and protein expression.

Jonathan C Trinidad1, Agnes Thalhammer, Christian G Specht

  • 1Mass Spectrometry Facility, Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143, USA.

Molecular & Cellular Proteomics : MCP
|December 7, 2007
PubMed
Summary

This study maps brain region-specific postsynaptic density (PSD) proteins and their phosphorylation. Findings reveal coordinated protein expression and differential receptor phosphorylation, highlighting the hippocampus

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Last Updated: Jul 9, 2026

Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence
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Quantifying Synapses: an Immunocytochemistry-based Assay to Quantify Synapse Number

Published on: November 16, 2010

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biochemistry

Background:

  • The postsynaptic density (PSD) is crucial for synaptic plasticity and function.
  • PSD protein composition varies across brain regions, influencing neuronal responses.
  • Understanding PSD molecular architecture is key to deciphering brain function.

Purpose of the Study:

  • To comprehensively compare protein expression and phosphorylation in PSDs from different murine brain regions.
  • To identify novel PSD components and understand regional differences in synaptic signaling.
  • To investigate the role of protein phosphorylation in regional synaptic strength modulation.

Main Methods:

  • Developed mass spectrometry-based methods for relative protein and phosphosite quantification.
  • Analyzed PSD preparations from murine cortex, midbrain, cerebellum, and hippocampus.
  • Utilized cluster analysis to identify functionally associated proteins and assess biological variability.

Main Results:

  • Quantified 2159 proteins and 1564 phosphorylation sites across four brain regions.
  • Identified coordinated expression of functionally linked proteins within PSDs.
  • Observed greater N-methyl-D-aspartate (NMDA) receptor phosphorylation than alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor phosphorylation.
  • Found highest kinase and phosphatase levels in the hippocampus, correlating with phosphopeptide abundance.

Conclusions:

  • PSD protein expression is coordinated and brain region-specific.
  • Differential phosphorylation of NMDA and AMPA receptors suggests distinct roles in synaptic processing.
  • The hippocampus exhibits a higher reliance on protein phosphorylation for synaptic strength regulation compared to other regions.