Related Experiment Video
Updated: Jul 9, 2026

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
Published on: November 7, 2012
Developments in directed evolution for improving enzyme functions
S Sen1, V Venkata Dasu, B Mandal
1Centre for the Environment, Indian Institute of Technology-Guwahati, Guwahati, Assam, 781039, India. shampa@iitg.ernet.in
Abstract:
The engineering of enzymes with altered activity, specificity, and stability, using directed evolution techniques that mimic evolution on a laboratory timescale, is now well established. In vitro recombination techniques such as DNA shuffling, staggered extension process (StEP), random chimeragenesis on transient templates (RACHITT), iterative truncation for the creation of hybrid enzymes (ITCHY), recombined extension on truncated templates (RETT), and so on have been developed to mimic and accelerate nature's recombination strategy. This review discusses gradual advances in the techniques and strategies used for the directed evolution of biocatalytic enzymes aimed at improving the quality and potential of enzyme libraries, their advantages, and disadvantages.
Related Concept Videos
Bioreactor Controls-III
Catalytically Perfect Enzymes
Evolution of New Traits in Microbes
Enzymes
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
Introduction to Mechanisms of Enzyme Catalysis
What is Genetic Engineering?

