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Updated: Jul 9, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
Ascorbate analogs for use in medical imaging: synthesis and radical scavenging activity of
Hiroharu Kato1, Tomohiro Kino, Fumihiko Yamamoto
1Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Abstract:
As part of our program to develop potential imaging agents for ascorbate bioactivity in the brain, 5-O-(4'-iodobenzyl)-L-ascorbic acid was prepared through a seven-step sequence which involved C5-O-alkylation with p-iodobenzyl bromide in the presence of Ag2O and CaSO4 as the key step, starting from L-ascorbic acid. The scavenging activity of the p-iodobenzylated analog against 2,2-diphenyl-1-picrylhyrazyl (DPPH) radical was almost the same as that of L-ascorbic acid itself.
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