Related Experiment Video
Updated: Jul 9, 2026
06:54
A Modified Inflammatory Pain Model to Study the Analgesic Effect in Mice
Published on: November 15, 2024
[Analysis of censored data in multi-factorial analgesic test]
Yojiro Sakiyama1, Osamu Komiyama, Hideo Tsukada
1Research Planning and Coordination, Nagoya Laboratories, Pfizer Global Research and Development, Pfizer Inc, 5-2 Taketoyo-cho, Chita-gun. Aichi 470-2393, Japan. Yojiro.Sakiyama@pfizer.com
Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan
|December 7, 2007
Summary
This study validates the tail withdrawal test for assessing animal pain sensitivity and analgesic drug efficacy. The findings highlight the importance of experimental design and statistical analysis for reliable results in pain research.
Area of Science:
- Neuroscience
- Pharmacology
- Animal Behavior
Context:
- Animal pain testing is crucial for developing novel analgesic drugs.
- The tail withdrawal experiment is a common method for assessing thermal pain sensitivity in animals.
- Proper experimental design and data analysis are essential for meaningful results.
Purpose:
- To evaluate the effect of water temperature on tail withdrawal latency in rhesus monkeys.
- To assess the impact of morphine on thermal sensitivity using the tail withdrawal test.
- To determine the reliability and reproducibility of the tail withdrawal assay for drug development.
Summary:
- The tail withdrawal experiment involved immersing rhesus monkey tails in warm water (38-40°C), with data censored at 120 seconds.
- A randomized block design and Friedman-type two-way analysis of variance (Mack-Skillings test) were employed.
- Significant effects of temperature and individual animals on withdrawal latency were observed, indicating inter-animal variability in thermal sensitivity.
Impact:
- The tail withdrawal test, combined with graphical analysis, offers a valuable method for evaluating experimental condition sensitivity and reproducibility.
- This approach can aid in assessing the pharmacological effects of analgesic drugs.
- The study underscores the importance of considering inter-animal differences in thermal sensitivity for robust pain research.
Related Concept Videos
01:09Censoring Survival Data
Survival analysis is a statistical method used to analyze time-to-event data, often employed in fields such as medicine, engineering, and social sciences. One of the key challenges in survival analysis is dealing with incomplete data, a phenomenon known as "censoring." Censoring occurs when the event of interest (such as death, relapse, or system failure) has not occurred for some individuals by the end of the study period or is otherwise unobservable, and it might have many different reasons...
02:07Blind Procedures
Ideally, the people who observe and record the children’s behavior are unaware of who was assigned to the experimental or control group, in order to control for experimenter bias. Experimenter bias refers to the possibility that a researcher’s expectations might skew the results of the study. Remember, conducting an experiment requires a lot of planning, and the people involved in the research project have a vested interest in supporting their hypotheses. If the observers knew which child was...
01:16Crossover Experiments
Crossover experiments, also called the repeated-measurements design, is a study design in which all experimental units are exposed to all treatments in different periods. Crossover experiments are generally used in psychology, the pharmaceutical industry, agriculture, and medicine.
Crossover designs are performed even with smaller sample sizes since the samples can act as their controls. These are better than simple randomized trials since patients are exposed to all the treatments.
Crossover designs are performed even with smaller sample sizes since the samples can act as their controls. These are better than simple randomized trials since patients are exposed to all the treatments.