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Published on: April 15, 2016
Experimental study on transarterial administration of GRGDSP combined with transarterial chemoembolization in rats
Jun Qian1, Jun Yin, Huimin Liang
1Department of Radiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, Peoples Republic of China. qianjun_hcc@yahoo.com
Purpose:
To evaluate the effects of transarterial administration of an integrin antagonist, GRGDSP (Gly-Arg-Gly-Asp-Ser-Pro), combined with transarterial chemoembolization (TACE) to treat hepatic carcinoma in rats.
Methods:
Walker-256 tumor was implanted beneath the liver capsule in 26 Wistar rats. Animal subjects were assigned to groups based on which treatment was injected into the hepatic artery: group A, GRGDSP + TACE; group B, TACE alone; and group C, normal saline. Magnetic resonance imaging (MRI), tumor pathology, and immunohistochemistry were performed to assess each treatment.
Results:
The ratios of the post-treatment to pretreatment tumor volumes (V2/V1) in groups A, B, and C were 4.42 +/- 0.48, 6.98 +/- 1.09, and 13.00 +/- 1.68, respectively. The metastatic potential of the tumors was assessed by tumor cell nest counts, which were 5.00 +/- 1.25, 6.63 +/- 1.60, and 7.22 +/- 1.92 in groups A, B, and C, respectively. Microvessel density (MVD) was quantified by measuring von Willebrand factor density values, which were 0.18 +/- 0.02, 0.22 +/- 0.02, and 0.23 +/- 0.02 in groups A, B, and C, respectively.
Conclusions:
Transarterial infusion of GRGDSP combined with TACE noticeably inhibited the growth of hepatic carcinoma and intrahepatic metastases in rats.
