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Intrastriatal Injection of Autologous Blood or Clostridial Collagenase as Murine Models of Intracerebral Hemorrhage
Published on: July 3, 2014
Preclinical models of intracerebral hemorrhage: a translational perspective
Michael Lucas James1, David S Warner, Daniel T Laskowitz
1Department of Anesthesiology, Duke University Medical Center, Box 3094, Durham, NC 27710, USA. james040@mc.duke.edu
Insights
Intracerebral hemorrhage (ICH) is a common, devastating brain bleed. This review examines preclinical models, highlighting their strengths and weaknesses for understanding ICH and developing new therapies.
Area of Science:
- Neurology
- Neurosurgery
- Translational Medicine
Background:
- Intracerebral hemorrhage (ICH) is a frequent and severe neurological condition with high mortality.
- Current treatments for ICH are limited, emphasizing the need for improved understanding and therapeutic strategies.
- Preclinical models are crucial for advancing ICH research, as highlighted by NIH and AHA reports.
Purpose of the Study:
- To review and evaluate various preclinical models used in intracerebral hemorrhage research.
- To analyze the strengths and weaknesses of different ICH model types across various species.
- To consider a mechanistic approach for understanding ICH pathophysiology and identifying therapeutic targets.
Main Methods:
- Review of established intracerebral hemorrhage models, including collagenase-induced hemorrhage and autologous blood injection.
- Examination of species utilized in different ICH model paradigms.
- Mechanistic evaluation of current knowledge and research breakthroughs in ICH.
Main Results:
- Traditional models like collagenase injection and blood infusion have inherent limitations and benefits.
- Different species and model types offer unique advantages for studying specific aspects of ICH.
- Transgenic rodent models show promise for investigating genetic factors influencing ICH recovery.
Conclusions:
- Understanding the strengths and weaknesses of each ICH model is vital for advancing research.
- Mechanistic insights from preclinical models can guide the development of novel therapeutic approaches for ICH.
- Further research utilizing diverse models, including transgenic rodents, is essential for improving outcomes in intracerebral hemorrhage patients.
Abstract:
Intracerebral hemorrhage (ICH) is a devastating and relatively common disease affecting as many as 50,000 people annually in the United States alone. ICH remains associated with poor outcome, and approximately 40-50% of afflicted patients will die within 30 days. In reports from the NIH and AHA, the importance of developing clinically relevant models of ICH that will extend our understanding of the pathophysiology of the disease and target new therapeutic approaches was emphasized. Traditionally, preclinical ICH research has most commonly utilized two paradigms: clostridial collagenase-induced hemorrhage and autologous blood injection. In this article, the use of various species is examined in the context of the different model types for ICH, and a mechanistic approach is considered in evaluating the numerous breakthroughs in our current fund of knowledge. Each of the model types has its inherent strengths and weaknesses and has the potential to further our understanding of the pathophysiology and treatment of ICH. In particular, transgenic rodent models may be helpful in addressing genetic influences on recovery from ICH.
