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Updated: Jul 9, 2026

Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection
Published on: January 10, 2012
In vitro incorporation of LNA nucleotides
Rakesh N Veedu1, Birte Vester, Jesper Wengel
1Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Odense M, Denmark. rnv@chem.sdu.dk
Abstract:
An LNA modified nucleoside triphosphate 1 was synthesized in order to investigate its potential to act as substrate for DNA strand synthesis by polymerases. Primer extension assays for the incorporation experiments revealed that Phusion High Fidelity DNA polymerase is an efficient enzyme for incorporation of the LNA nucleotide and for extending strand to full length. It was also observed that pfu DNA polymerase could incorporate the LNA nucleotide but it failed to extend the strand to a full length product.
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