Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Proteinase inhibition using small Bowman-Birk-type structures.

J H Fernandez¹, M O Mello, L Galgaro

¹LabInfo, LNCC, Petrópolis, RJ, Brasil.

Genetics and Molecular Research : GMR

|December 7, 2007

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A novel Kazal-type protease inhibitor from Aedes aegypti effective for NS2B-NS3 protease from the Zika virus and subtilases.

Archives of biochemistry and biophysics·2026

Same author

The gut microbiota composition of Trichoplusia ni is altered by diet and may influence its polyphagous behavior.

Scientific reports·2021

Same author

Soybean leghemoglobin targeted to potato chloroplasts influences growth and development of transgenic plants.

Plant cell reports·2019

Same author

Novel pseudo-aspartic peptidase from the midgut of the tick Rhipicephalus microplus.

Scientific reports·2019

Same author

Selection and validation of reference house-keeping genes in the J774A1 macrophage cell line for quantitative real-time PCR.

Genetics and molecular research : GMR·2016

Same author

EPI-peptide designer: a tool for designing peptide ligand libraries based on epitope-paratope interactions.

Bioinformatics (Oxford, England)·2016

Same journal

Diallel crosses for resistance to Macrophomina phaseolina and Thanatephorus cucumeris on cowpea.

Genetics and molecular research : GMR·2017

Same journal

Diversity among elephant grass genotypes using Bayesian multi-trait model.

Genetics and molecular research : GMR·2017

Same journal

Coupled transcript and metabolite identification: insights on induction and synthesis of resveratrol in peanut, wild relatives and synthetic allotetraploid.

Genetics and molecular research : GMR·2017

Same journal

Estimates of genetics and phenotypics parameters for the yield and quality of soybean seeds.

Genetics and molecular research : GMR·2017

Same journal

In silico modeling and characterization of phytoparasitic nematodes translationally-controlled tumor proteins.

Genetics and molecular research : GMR·2017

Same journal

Molecular identification of variety purity in a cotton hybrid with unknown parentage using DNA-SSR markers.

Genetics and molecular research : GMR·2017

See all related articles

Bowman-Birk inhibitors (BBIs) are small proteins that inhibit digestive enzymes. Computational studies revealed a mutant BBI (ChyTB2) effectively inhibits both trypsin and chymotrypsin through distinct binding interactions.

Area of Science:

Biochemistry
Structural Biology
Computational Biology

Background:

Bowman-Birk inhibitors (BBIs) are small, cysteine-rich proteins that inhibit digestive proteinases.
BBIs possess a double-headed structure with independent loops for binding trypsin, chymotrypsin, and elastase.

Purpose of the Study:

To investigate the structural characteristics and inhibition dynamics of a modified BBI loop (ChyTB2 inhibitor).
To analyze the binding interactions of ChyTB2 with trypsin and chymotrypsin using computational methods.

Main Methods:

Computational biology techniques, including molecular modeling and dynamics simulations.
Enzyme-linked immunosorbent assay (ELISA) to validate computational findings.

Main Results:

Related Experiment Videos

BBI-trypsin inhibition involves salt bridges and hydrogen bonds.
BBI-chymotrypsin inhibition is primarily driven by hydrophobic interactions.
The ChyTB2 mutant maintained its structure and effectively inhibited both trypsin and chymotrypsin.

Conclusions:

Computational modeling and molecular dynamics are effective for predicting and designing novel proteinase inhibitors.
Designing small BBI-type inhibitors requires understanding the specific binding interactions of mutants with target proteinases.