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c-Maf expression in angioimmunoblastic T-cell lymphoma.
Yoshiko Idate Murakami1, Yasushi Yatabe, Teruhiro Sakaguchi
1Department of Pathology of Biological Response, Nagoya University Graduate School of Medicine, Tsurumai-cho 65, Showa-ku, Japan. yidate@med.nagoya-u.ac.jp
The American Journal of Surgical Pathology
|December 7, 2007
Summary
The oncogene c-Maf is highly expressed in angioimmunoblastic T-cell lymphoma (AILT), often alongside cyclins D1 and D2. This suggests c-Maf may play a role in AILT pathogenesis and could serve as a diagnostic marker.
Area of Science:
- Oncology
- Immunology
- Molecular Biology
Background:
- The oncogene c-Maf is overexpressed in multiple myeloma and its role in cyclin D2 expression is postulated.
- Previous studies indicated extremely elevated c-Maf levels in angioimmunoblastic T-cell lymphoma (AILT) via reverse-transcription polymerase chain reaction.
Purpose of the Study:
- To investigate c-Maf and cyclin expression in various T-cell lymphomas using immunohistochemistry.
- To explore the potential of c-Maf as a diagnostic marker for AILT.
Main Methods:
- Immunohistochemical analysis of c-Maf and cyclin expression in 93 T-cell lymphoma cases.
- Double immunostaining to identify cell surface markers on c-Maf-positive cells in AILT.
- Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) to confirm c-Maf overexpression.
Main Results:
- c-Maf expression was detected in 23/31 cases of AILT, significantly higher than in other T-cell lymphomas.
- In AILT, c-Maf-positive cells were predominantly CD4-positive, CD43, and CD45RO, but negative for CD20.
- Cyclins D1 and D2 were overexpressed in many c-Maf-positive AILT samples. qRT-PCR confirmed c-Maf overexpression in 8/31 AILT cases.
Conclusions:
- CD4-positive neoplastic T cells in AILT exhibit c-Maf expression.
- These findings provide new insights into AILT pathogenesis.
- c-Maf is suggested as a potential diagnostic marker for AILT.
