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Phase II Reactions: Methylation Reactions01:17

Phase II Reactions: Methylation Reactions

Methylation is a phase II biotransformation process involving the attachment of a methyl group to a substrate. Enzymes known as methyltransferases orchestrate this reaction.
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Related Experiment Video

Updated: Jul 9, 2026

Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques
11:49

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Published on: March 24, 2016

Structure, function, and evolution of plant O-methyltransferases.

Kevin C Lam1, Ragai K Ibrahim, Bahareh Behdad

  • 1Biology Department, Concordia University, 7141 Sherbrooke Street West, Montréal, QC H4B 1R6, Canada.

Genome
|December 7, 2007
PubMed
Summary
This summary is machine-generated.

Plant O-methyltransferases (OMTs) are crucial enzymes in plant metabolism. Phylogenetic analysis reveals their evolutionary relationships, aiding in the functional prediction of new OMT genes.

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Area of Science:

  • Biochemistry
  • Plant Biology
  • Enzymology

Background:

  • Plant O-methyltransferases (OMTs) are a large enzyme family involved in methylating diverse secondary metabolites.
  • O-Methylation is vital for plant processes like lignin biosynthesis, stress tolerance, and disease resistance.

Purpose of the Study:

  • To investigate the evolutionary diversity of plant O-methyltransferases.
  • To construct a phylogenetic tree for predicting the functions of novel OMT-like genes.

Main Methods:

  • Comparative and phylogenetic analysis of 61 biochemically characterized plant OMT protein sequences.

Main Results:

  • The phylogenetic tree identified two major OMT groups, including caffeoyl CoA OMTs (CCoA OMTs) and carboxylic acid OMTs.
  • Analysis suggests both convergent and divergent evolution within the OMT family.
  • Conserved regions in CCoA OMTs and carboxylic acid OMTs can be used to design primers for gene characterization.

Conclusions:

  • The study provides a phylogenetic framework for understanding plant OMT evolution and function.
  • Identified conserved regions offer a tool for discovering and characterizing new OMT genes across plant species.