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Related Concept Videos

Computed Tomography01:10

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...

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Related Experiment Video

Updated: Jul 9, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
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Parallel optical coherence tomography system.

Yuan Luo1, Lina J Arauz, Jose E Castillo

  • 1Department of Electrical and Computer Engineering, University of Arizona, 1230 E. Speedway Blvd, Tucson, AZ 85721, USA. luo@email.arizona.edu

Applied Optics
|December 7, 2007
PubMed
Summary

We developed a parallel optical coherence tomography (POCT) system for endoscopy. This electronic scanning system achieves high lateral resolution comparable to mechanical systems.

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Area of Science:

  • Biomedical Optics
  • Medical Imaging
  • Endoscopic Technologies

Background:

  • Optical Coherence Tomography (OCT) is a valuable non-invasive imaging modality.
  • Traditional OCT systems often rely on mechanical scanning, limiting speed and complexity.
  • Endoscopic OCT requires miniaturized and efficient scanning mechanisms.

Purpose of the Study:

  • To design and implement a parallel optical coherence tomography (POCT) imaging system.
  • To adapt the POCT system for endoscopic applications.
  • To evaluate the performance of the POCT system in terms of speed and resolution.

Main Methods:

  • Utilized a single mode fiber (SMF) array with reduced diameter (15 microm) SMFs.
  • Employed an array with 15 microm center spacing for transverse imaging field control.
  • Implemented electronic scanning to eliminate lateral mechanical scanning.
  • Acquired experimental image data using the developed POCT system.

Main Results:

  • Demonstrated parallel axial scan acquisition capability.
  • Achieved lateral resolution comparable to mechanically scanned OCT systems.
  • The system's transverse imaging field is scalable by adjusting the array size.

Conclusions:

  • The developed POCT system offers a viable alternative to mechanical scanning in OCT.
  • The electronic scanning approach enhances imaging speed and simplifies endoscopic integration.
  • This technology holds promise for advanced endoscopic imaging applications.