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Updated: Jul 9, 2026
11:31
Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
Published on: July 4, 2018
Mast cell reaction in malignant laryngeal neoplasm
N Balica1, M Raica, S Cotulbea
1ENT Department, Victor Babes University of Medicine and Pharmacy, Timişoara, Romania. nic_balica@yahoo.com
Summary
Mast cells are more numerous in early-stage laryngeal cancers but decrease in later stages. This study investigated mast cell density in various laryngeal neoplasms.
Area of Science:
- Oncology
- Histopathology
- Cell Biology
Background:
- Mast cells are common in respiratory tissues, and their hyperplasia is observed in malignant tumors.
- The role of mast cells in laryngeal cancer remains poorly understood.
- Limited data exists on mast cell reactions within malignant laryngeal neoplasms.
Purpose of the Study:
- To investigate the density and distribution of mast cells in laryngeal carcinomas.
- To correlate mast cell presence with different stages and types of laryngeal cancer.
Main Methods:
- Histological analysis of 127 laryngeal carcinoma archive blocks.
- Hematoxylin-Eosin and Alcian blue-Safranin histochemistry for mast cell identification.
- Microscopic examination and image analysis using Lucia G software.
Main Results:
- Squamous cell carcinoma was the predominant type (G1-G3: 99.5%).
- Mast cell microdensity was higher in microinvasive squamous cell carcinoma (4.66) compared to invasive (2.19).
- Significantly higher mast cell microdensities were noted in malignant laryngeal papillomatosis (9.33) and adenoid cystic carcinoma (46.66).
Conclusions:
- Mast cell density is higher in early-stage laryngeal cancers and diminishes in advanced stages.
- Alcian blue-positive mast cells are found within the tumor, while safranin-positive mast cells are located in surrounding connective and muscular tissues.
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