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Updated: Jul 9, 2026

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
Published on: May 3, 2018
Identification and characterization of cyclin X which activates transcriptional activities of c-Myc
Xuanyang Li1, Xiang Wang, Guoyuan Liu
1Institute of Genetics, School of Life Science, Fudan University, Shanghai, 200433, People's Republic of China.
Abstract:
Cyclins are eukaryotic proteins that play an active role in controlling cell division cycles and regulate cyclin dependent kinases (CDKs). In this study, we identified and characterized a new member of the human cyclin family. Its cDNA sequence contains an open reading frame of 864 bp, encoding a protein of 287 amino acids. This putative cyclin, designated cyclin X, contains a typical cyclin box. Cyclin X shows the highest sequence similarity in cyclin box to cyclin B3 and the similarity between these two proteins also extends to the whole molecule. The expression pattern of cyclin X showed that it was highly expressed in testis. Cyclin X protein was located in nucleus of the H1299 cells and Overexpression of cyclin X activated the transcriptional activities of c-Myc. These results indicate that cyclin X is a novel member of the human cyclin family and positive regulator of c-Myc-mediated gene transcription.
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