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Updated: Jul 9, 2026

Optimizing Isolation and Purification of Murine Glomerular Mesangial Cells
Published on: March 7, 2025
Glutamine enhances glucose-induced mesangial cell proliferation
Claudia J Lagranha1, Sonia Q Doi, Tania C Pithon-Curi
1Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, Avenida Professor Lineu Prestes 1524, 05508-900 São Paulo, SP, Brazil. lagranha@hotmail.com
Abstract:
The proliferation of mesangial cells (MC) in the presence of glutamine (0-20 mM) was determined in both low (5 mM) and high (25 mM) glucose-containing medium. Glutamine in a high glucose (HG) environment increased cell proliferation in a dose-dependent manner. Inhibition of glutamine:fructose 6-phosphate amidotransferase (GFAT) and of phosphodiesterase significantly reduced glutamine-induced proliferation. Supraphysiologic levels of glutamine increase MC proliferation in a HG milieu via GFAT and cAMP-dependent pathways, suggesting that glutamine could pose a risk for diabetic nephropathy.
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