Related Experiment Video
Updated: Jul 9, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Published on: August 29, 2014
A new 16S rDNA-RFLP method for the discrimination of the accepted species of Arcobacter
Maria José Figueras1, Luis Collado, Josep Guarro
1Unitat de Microbiologia, Departament de Ciències Mèdiques Bàsiques, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Sant Llorenç 21, Reus, Spain. mariajose.figueras@urv.cat
Abstract:
The genus Arcobacter that includes some species of clinical interest has been recently enlarged with the addition of several new species. The available molecular techniques for the characterization of Arcobacter spp. are unable to identify all the species included in this genus. We have developed a 16S rDNA-RFLP method able to separate the currently accepted 6 species of Arcobacter, including the 2 hybridization groups of Arcobacter cryaerophilus. The method based on the use of a pair of primers and of the endonuclease MseI has been validated using 12 reference strains (including the type strains) and 75 fresh isolates. All isolates tested produced species-specific RFLP patterns. This easy-to-perform method allows a fast and reliable recognition of the members of this complex genus.
More Related Videos
Related Concept Videos
Applications of Molecular Taxonomy
Modern Molecular Taxonomy
Phylogenetic Species Concept in Microbiology
Rapid Identification of Pathogens

