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Related Concept Videos

The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
Microtubule Instability02:17

Microtubule Instability

Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated assembly and...
Microtubule Instability02:17

Microtubule Instability

Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated assembly and...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...

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Related Experiment Video

Updated: Jul 9, 2026

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization
08:52

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Published on: August 16, 2015

Cell-cycle progression without an intact microtuble cytoskeleton.

Yumi Uetake1, Greenfield Sluder

  • 1Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.

Current Biology : CB
|December 7, 2007
PubMed
Summary
This summary is machine-generated.

Microtubule integrity is not essential for human cell cycle progression through G1. Cells lacking a microtubule cytoskeleton after mitosis can still enter and complete interphase normally.

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Last Updated: Jul 9, 2026

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization
08:52

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Published on: August 16, 2015

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
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Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry
11:23

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

Published on: May 22, 2012

Area of Science:

  • Cell Biology
  • Cytoskeleton Dynamics
  • Cell Cycle Regulation

Background:

  • The role of microtubule cytoskeleton integrity in mammalian somatic cell interphase progression remains unclear.
  • Previous studies suggested a "microtubule integrity" checkpoint causing G1 arrest upon microtubule disruption.

Purpose of the Study:

  • To investigate the necessity of microtubule cytoskeleton integrity for cell cycle progression in human somatic cells.
  • To determine if a checkpoint surveils microtubule integrity after mitosis.

Main Methods:

  • Human cells (hTERT RPE1 and primary fibroblasts) were treated with nocodazole or colcemid to disassemble microtubules.
  • Microtubules were rapidly disassembled in cells exiting mitosis of normal duration by chilling.
  • Cells with diminished, absent, or stabilized microtubules were analyzed for cell cycle progression.

Main Results:

  • Cells lacking microtubules after a normal mitosis progressed through G1 and interphase with normal kinetics.
  • Partial microtubule reduction or stabilization with taxol did not impede cell cycle progression.
  • Prolonged mitotic arrest followed by G1 arrest was observed with continuous nocodazole exposure, but this was bypassed by isolating cells exiting normal mitosis.

Conclusions:

  • Microtubule cytoskeleton integrity is not monitored by a checkpoint after mitosis.
  • Normal human cells can progress through G1 and interphase without an intact microtubule cytoskeleton.