Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

α-Hydroxy Ketones via Reductive Coupling of Esters: Acyloin Condensation Overview01:19

α-Hydroxy Ketones via Reductive Coupling of Esters: Acyloin Condensation Overview

The pinacol and McMurry reactions involve the reductive coupling of ketones or aldehydes. Similarly, the bimolecular reductive coupling of two ester molecules in the presence of sodium metal in an aprotic solvent yields an α-hydroxy ketone product. The α-hydroxy ketone is also called acyloin, so the reaction is referred to as ‘acyloin condensation.’
Oxidation of Alkenes: Syn Dihydroxylation with Osmium Tetraoxide02:44

Oxidation of Alkenes: Syn Dihydroxylation with Osmium Tetraoxide

Alkenes are converted to 1,2-diols or glycols through a process called dihydroxylation. It involves the addition of two hydroxyl groups across the double bond with two different stereochemical approaches, namely anti and syn. Dihydroxylation using osmium tetroxide progresses with syn stereochemistry.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Antiproliferative Activity and NO Inhibition of <i>Neo</i>-Clerodane Diterpenoids from <i>Salvia guevarae</i> in RAW 264.7 Macrophages.

Molecules (Basel, Switzerland)·2025
Same author

Structure, Absolute Configuration, Antiproliferative and Phytotoxic Activities of Icetexane and Abietane Diterpenoids from <i>Salvia carranzae</i> and Chemotaxonomic Implications.

Molecules (Basel, Switzerland)·2024
Same author

Molecular orbital and topological electron density study of n → π* interactions: amides and thioamides cases.

RSC advances·2023
Same author

Effect of extracts from several sponges of Yucatan Coast on <i>Giardia lamblia</i> and preliminary chemical investigation of the bioactive extract of <i>Haliclona</i> (<i>Reinera</i>) <i>tubifera</i>.

Natural product research·2022
Same author

The origin of the regiospecificity of acrolein dimerization.

RSC advances·2022
Same author

Microwave-enhanced synthesis of 26-amino-22-oxocholestanes and their cytotoxic activity.

Steroids·2022

Related Experiment Video

Updated: Jul 9, 2026

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
08:56

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

21beta-Hydroxy-oleanane-type triterpenes from Hippocratea excelsa.

David Cáceres-Castillo1, Gonzalo J Mena-Rejón, Roberto Cedillo-Rivera

  • 1Laboratorio de Química Orgánica de Investigación, Facultad de Química, Universidad Autónoma de Yucatán, Calle 41 No. 421, Col. Industrial, C.P. 97150 Mérida, Yucatán, México.

Phytochemistry
|December 7, 2007
PubMed
Summary

Six new pentacyclic triterpenes were isolated from Hippocratea excelsa stem bark. These compounds, along with known substances, were identified using spectroscopic methods, but showed no significant antigiardial activity.

More Related Videos

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

Related Experiment Videos

Last Updated: Jul 9, 2026

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
08:56

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

Area of Science:

  • Phytochemistry
  • Natural Products Chemistry
  • Organic Chemistry

Background:

  • Hippocratea excelsa is a plant species known for its potential medicinal properties.
  • Pentacyclic triterpenes are a class of natural compounds with diverse biological activities.
  • Previous studies have investigated compounds from the root bark of Hippocratea excelsa.

Purpose of the Study:

  • To isolate and characterize pentacyclic triterpenes from the stem bark of Hippocratea excelsa.
  • To evaluate the antigiardial activity of the isolated compounds.

Main Methods:

  • Extraction and isolation of compounds from plant material.
  • Structure elucidation using advanced spectroscopic techniques, including Nuclear Magnetic Resonance (NMR) spectroscopy (COSY, ROESY, HSQC, HMBC).
  • Comparison of spectral data with existing literature.

Main Results:

  • Six novel pentacyclic triterpenes (five oleanane-type and one ursane-type) were identified from the stem bark.
  • Previously isolated compound 3alpha,21beta-dihydroxy-olean-12-ene (1) was also found.
  • Known compounds including alpha- and beta-amyrin, oleanoic acid, ursolic acid, trans-polyisoprene, and beta-sitosterol were isolated.
  • Compounds 2-5 demonstrated no significant antigiardial activity.

Conclusions:

  • The stem bark of Hippocratea excelsa is a source of diverse pentacyclic triterpenes.
  • The spectroscopic characterization confirmed the structures of the isolated compounds.
  • The evaluated compounds did not exhibit significant efficacy against Giardia.