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Related Experiment Video

Updated: Jul 9, 2026

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
14:48

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

Proteomic methods for biomarker discovery in urine.

Daniel W Wilkey1, Michael L Merchant

  • 1Kidney Disease Program, University of Louisville, Louisville, KY 40202, USA.

Seminars in Nephrology
|December 7, 2007
PubMed
Summary

Proteomic methods are advancing biomarker discovery for kidney diseases, overcoming challenges like sample issues and protein degradation. These advancements aid in understanding disease progression and guiding patient treatment.

Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Area of Science:

  • Nephrology
  • Proteomics
  • Biomarker Discovery

Background:

  • Biomarker identification for kidney diseases faces challenges including sample heterogeneity, workflow issues, and protein degradation.
  • Despite these hurdles, proteomic techniques have shown significant progress in renal disease research.

Purpose of the Study:

  • To highlight the progress and potential of proteomic methods in identifying biomarkers for renal diseases.
  • To discuss the application of proteomics in understanding disease mechanisms and guiding therapeutic strategies.

Main Methods:

  • Electrophoretic, chromatographic, and mass spectrometric methods are key proteomic techniques.
  • Analysis of protein expression profiles in complex renal diseases.

Main Results:

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A Modified Precipitation Method to Isolate Urinary Exosomes
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A Modified Precipitation Method to Isolate Urinary Exosomes

Published on: January 16, 2015

Related Experiment Videos

Last Updated: Jul 9, 2026

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
14:48

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

A Modified Precipitation Method to Isolate Urinary Exosomes
05:05

A Modified Precipitation Method to Isolate Urinary Exosomes

Published on: January 16, 2015

  • Proteomics has identified novel mechanisms in renal disease progression.
  • Established protein expression profiles for glomerular and tubular pathologies.
  • Identified potential biomarkers for guiding patient treatment and pharmacologic therapies.

Conclusions:

  • Proteomic analysis, though recent in renal disease study, shows substantial potential.
  • Advances in proteomics offer new avenues for diagnosing and managing kidney diseases.