Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

M-Cdk Drives Transition Into Mitosis

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...

Mitogens and the Cell Cycle

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Role of channels in the O<sub>2</sub> permeability of murine red blood cells II. Morphological and proteomic studies.

bioRxiv : the preprint server for biology·2025

Same author

A pharmacodynamic assay to monitor treatment with the hypomethylating cytosine analogs, decitabine and azacitidine.

Methods in cell biology·2024

Same author

Analysis of the multiparametric cell cycle data.

Methods in cell biology·2024

Same author

Flow cytometry of DNMT1 as a biomarker of hypomethylating therapies.

Cytometry. Part B, Clinical cytometry·2024

Same author

Duffy antigen is expressed during erythropoiesis in Duffy-negative individuals.

Cell host & microbe·2023

Same author

Pharmacokinetics and pharmacodynamics of an oral formulation of decitabine and tetrahydrouridine.

European journal of haematology·2023

Search research articles

Related Experiment Video

Updated: Jul 9, 2026

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

A new biomarker for mitotic cells.

James W Jacobberger¹, Phyllis S Frisa, R Michael Sramkoski

¹Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, Ohio 44106, USA. jwj@case.edu

Cytometry. Part a : the Journal of the International Society for Analytical Cytology

|December 7, 2007

Summary

A novel antibody targeting the phospho-serine 780 epitope of retinoblastoma protein (pS780-Rb) effectively identifies mitotic cells. This robust marker aids in cell cycle analysis and counting mitotic and newborn cells.

More Related Videos

Live Cell Imaging of Chromosome Segregation During Mitosis

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations

Published on: September 17, 2012

Related Experiment Videos

Last Updated: Jul 9, 2026

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

Live Cell Imaging of Chromosome Segregation During Mitosis

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations

Published on: September 17, 2012

Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

Phosphorylated epitopes serve as crucial biomarkers for identifying cells undergoing mitosis.
Commonly used markers include MPM-2 and histone H3 phosphorylated at serine 10.
The retinoblastoma protein (Rb) is a key regulator of the cell cycle.

Purpose of the Study:

To evaluate a novel antibody targeting a specific phosphorylated epitope on the retinoblastoma protein (phospho-S780-Rb) as a mitotic cell marker.
To compare the efficacy of this new marker with established mitotic markers like MPM-2 and phospho-histone H3.
To assess the utility of phospho-S780-Rb in detailed cell cycle analysis.

Main Methods:

Human cell lines were stained with DNA dyes and antibodies against MPM-2, phospho-S10-histone-H3, and phospho-S780-Rb.
Immunoreactivity and DNA content were analyzed using flow and image cytometry.
Western blotting, immunoprecipitation, and bromodeoxyuridine (BrdU) costaining were employed to characterize the epitope.

Main Results:

Phospho-S780-Rb immunofluorescence strongly correlated with MPM-2 reactivity and was expressed throughout all stages of mitosis.
The epitope was confirmed to be present on the Rb protein and other peptides and demonstrated acid stability.
Kinetic experiments highlighted the marker's utility in precise cell cycle transition state timing.

Conclusions:

The phospho-S780-Rb epitope (pS780) is a reliable and robust marker for cytometric detection of mitotic cells, from chromatin condensation through cytokinesis.
This marker facilitates the discrimination and counting of mitotic and post-cytokinetic cells when used with DNA dyes.
pS780 offers a valuable tool for complex cell cycle analyses and understanding cell division dynamics.