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Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems
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Representational fragment amplification: exponential amplification of fragmented cDNA enables multimillion-fold

Gregory D Sgarlato1, Howard H Sussman

  • 1Department of Pathology, Stanford University, Stanford, CA, USA.

Clinical Chemistry
|December 7, 2007
PubMed
Summary

No abstract available in PubMed .

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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
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