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Updated: Jul 9, 2026
DNA Vector-based RNA Interference to Study Gene Function in Cancer
Published on: June 4, 2012
[Construction of an expression vector for high expression of siRNA]
Hui-Jin Li1, Xiao-Jing Zheng, Wei-Feng Zhang
1College of Life Sciences, Shaanxi Normal University, Xi'an 710100, China. lihuijin@stu.snnu.edu.cn
Aim:
To construct the vector for efficient expression of siRNA using pre-mir30 backbone.
Methods:
By chemical synthesis method, pre-mir30 backbone introduced an appropriate restriction enzyme site for foreign shRNA inserting was cloned into an expressing vector containing U6 promoter. The silencing efficiency of a new siRNA expressing vector was detected by transfection and Western blot.
Results:
The new vector containing pre-mir30 backbone expressing siRNA against GFP could markedly inhibit the expression of GFP compared with the vector expressing control siRNA.
Conclusion:
siRNA expressing vector constructed by pre-mir30 backbone could highly express foreign siRNA.
