Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Ultrasonic baths as substitutes for shaking incubator baths.

N S Radin¹, G S Shukla

¹Mental Health Research Institute, University of Michigan, Ann Arbor.

Enzyme

|January 1, 1991

Summary

Researchers developed an affordable, compact enzyme incubation bath using an ultrasonic cleaner and temperature controller. This novel device offers superior mixing for enzyme assays, especially with complex biological samples.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Multiple antibodies targeting tumor-specific mutations redirect immune cells to inhibit tumor growth and increase survival in experimental animal models.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2019

Same author

Maternal cadmium intoxication: effects on fetal brain antioxidant defense parameters.

Redox report : communications in free radical research·2016

Same author

Combination treatment with Grb7 peptide and Doxorubicin or Trastuzumab (Herceptin) results in cooperative cell growth inhibition in breast cancer cells.

British journal of cancer·2007

Same author

Infections and glycolipids.

Postgraduate medical journal·2003

Same author

Apoptotic death by ceramide: will the real killer please stand up?

Medical hypotheses·2001

Same author

Cadmium-mediated oxidative stress in alveolar epithelial cells induces the expression of gamma-glutamylcysteine synthetase catalytic subunit and glutathione S-transferase alpha and pi isoforms: potential role of activator protein-1.

Cell biology and toxicology·2001

Area of Science:

Biochemistry
Enzymology
Laboratory Equipment Design

Background:

Enzyme assays require precise temperature control and effective sample mixing.
Commercially available shaking baths can be expensive, noisy, and bulky.
Heterogeneous samples, such as those with tissue homogenates, pose mixing challenges.

Purpose of the Study:

To describe the assembly and function of a novel, cost-effective incubation bath for enzyme work.
To compare the performance of the new device against traditional shaking baths.

Main Methods:

The incubation bath was constructed using a standard ultrasonic cleaning bath and a separate temperature-controlling device.
Assembly involved integrating these commercially available units.
Performance was evaluated based on mixing efficiency, particularly for heterogeneous samples.

Main Results:

The assembled incubation bath is significantly cheaper, quieter, and more compact than commercial shaking baths.
It provides superior mixing of heterogeneous enzyme incubation samples.
The device effectively handles samples containing tissue homogenates or subcellular particles.

Conclusions:

A simple, economical, and efficient incubation bath can be assembled from readily available components.
This device offers a practical alternative for enzyme work, especially in resource-limited settings.
The ultrasonic-based design enhances mixing for challenging biological sample types.

Related Experiment Videos