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Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry
Published on: November 25, 2011
Photodynamic effect on melanoma cells investigated by atomic force microscopy
K Tomankova1, H Kolarova, M Vujtek
1Department of Medical Biophysics, Faculty of Medicine, Palacky University in Olomouc, Hnevotínská 3, 775 15 Olomouc, Czech Republic. tomanko@tunw.upol.cz
General Physiology and Biophysics
|December 8, 2007
Summary
Atomic force microscopy (AFM) revealed significant changes in G361 cell surfaces after photodynamic effect (PDE) induction. Imaging showed a transition from smooth surfaces to rough, protruding, and cleaved structures, demonstrating AFM
Area of Science:
- Biophysics
- Cell Biology
- Materials Science
Background:
- Atomic force microscopy (AFM) enables high-resolution imaging of biological samples.
- Scanning probe microscopy (SPM) allows live cell imaging in various environments.
- Observing cellular morphology changes is crucial for understanding biological processes.
Purpose of the Study:
- To image the surface of G361 cells in air using AFM.
- To investigate morphological changes in G361 cells before and after photodynamic effect (PDE) induction.
Main Methods:
- Utilized AFM with a dry scanner in non-contact or tapping mode.
- Integrated an inverse optical microscope for precise scanning tip positioning.
- Imaged G361 cell line before and after PDE induction via ZnTPPS4 irradiation (15 J/cm(2)).
Main Results:
- G361 cells exhibited a smooth surface without protrusions prior to PDE induction.
- Following PDE induction, cells displayed a rough surface with noticeable protrusions and cleavage.
- AFM successfully visualized distinct surface alterations induced by the photodynamic effect.
Conclusions:
- AFM is effective for visualizing subtle changes in cell surface topography.
- Photodynamic effect significantly alters G361 cell surface morphology.
- This imaging approach provides insights into cellular responses to treatment.
