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Updated: Jul 9, 2026
04:56
Determination of the Procoagulant Activity of Extracellular Vesicle (EV) Using EV-Activated Clotting Time (EV-ACT)
Published on: August 4, 2023
Plasma cholesteryl ester transfer protein and blood coagulability
Hiroshi Deguchi1, José A Fernández, John H Griffin
1Dept. of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037, USA.
Thrombosis and Haemostasis
|December 8, 2007
Summary
Low high density lipoprotein (HDL) levels are linked to venous thrombosis. This study found that cholesteryl ester transfer protein (CETP) plasma levels positively correlate with blood coagulability, potentially increasing thrombotic event risk.
Area of Science:
- Biochemistry
- Hematology
- Lipid Metabolism
Background:
- Low high density lipoprotein (HDL) levels are associated with venous thrombosis in young men and recurrence in patients with prior unprovoked episodes.
- Cholesteryl ester transfer protein (CETP) influences HDL metabolism, and lipoproteins can impact blood coagulation.
- The relationship between CETP levels and blood coagulability requires further investigation.
Purpose of the Study:
- To investigate the association between cholesteryl ester transfer protein (CETP) mass and blood coagulability.
- To determine if CETP influences coagulation pathways and prothrombinase activity.
Main Methods:
- Plasma samples from 39 healthy adults were analyzed for CETP mass and coagulability.
- Clotting times were measured using dilute tissue factor or factor XIa.
- The effect of recombinant CETP on factor Xa-1-stage clotting times and prothrombin activation was assessed.
Main Results:
- Clotting times significantly correlated with CETP antigen levels for both extrinsic (tissue factor) and intrinsic (factor XIa) pathways.
- Recombinant CETP shortened factor Xa-1-stage clotting times, indicating augmented procoagulant activity.
- Recombinant CETP dose-dependently increased prothrombin activation, suggesting enhanced prothrombinase activity.
Conclusions:
- Plasma CETP levels are positively correlated with blood coagulability.
- CETP may enhance prothrombinase activity, contributing to increased thrombotic risk.
- This finding reveals a novel link between CETP and coagulation, potentially relevant to thrombotic events.
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