Bottom-up genome assembly using the Bacillus subtilis genome vector
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Updated: Jul 9, 2026

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The MultiBac Protein Complex Production Platform at the EMBL
Published on: July 11, 2013
Mitsuhiro Itaya1, Kyoko Fujita, Azusa Kuroki
1Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida-shi, Tokyo 194-8511, Japan. mita2001@sfc.keio.ac.jp
Nature Methods
|December 11, 2007
Abstract:
We established a protocol to construct complete recombinant genomes from their small contiguous DNA pieces and obtained the genomes of mouse mitochondrion and rice chloroplast using a B. subtilis genome (BGM) vector. This method allows the design of any recombinant genomes, valuable not only for fundamental research in systems biology and synthetic biology but also for various applications in the life sciences.

