Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Time-resolved DNA-microarray reading by an intensified CCD for ultimate sensitivity.

G Valentini, C D'Andrea, D Comelli

    Optics Letters
    |December 11, 2007
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Simvastatin ameliorates senescence-induced mitochondrial dysfunction in vascular smooth muscle cells.

    Atherosclerosis·2025
    Same author

    Antiprotons and Elementary Particles over a Solar Cycle: Results from the Alpha Magnetic Spectrometer.

    Physical review letters·2025
    Same author

    Solar Modulation of Cosmic Nuclei over a Solar Cycle: Results from the Alpha Magnetic Spectrometer.

    Physical review letters·2025
    Same author

    A personalized clinical assessment: multi-sensor approach for understanding musculoskeletal health in the frail population.

    Biomedical engineering online·2024
    Same author

    AIEC-dependent pathogenic Th17 cell transdifferentiation in Crohn's disease is suppressed by <i>rfaP</i> and <i>ybaT</i> deletion.

    Gut microbes·2024
    Same author

    Properties of Cosmic Deuterons Measured by the Alpha Magnetic Spectrometer.

    Physical review letters·2024
    Same journal

    Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

    Optics letters·2026
    Same journal

    E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

    Optics letters·2026
    Same journal

    Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

    Optics letters·2026
    Same journal

    Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

    Optics letters·2026
    Same journal

    Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

    Optics letters·2026
    Same journal

    Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

    Optics letters·2026
    See all related articles

    This study introduces a new DNA microarray reading method using time-resolved fluorescence. The technique enhances the detection of hybridized DNA spots, improving accuracy in genetic analysis.

    Area of Science:

    • Biotechnology
    • Molecular Biology
    • Analytical Chemistry

    Background:

    • DNA microarrays are crucial for genetic analysis.
    • Current detection methods can miss subtle hybridization signals.
    • Need for enhanced sensitivity in DNA microarray reading.

    Purpose of the Study:

    • To develop and present a novel time-resolved fluorescence technique for DNA microarray reading.
    • To improve the detection of hybridized DNA spots.
    • To overcome limitations of continuous-wave detection methods.

    Main Methods:

    • Utilized an intensified CCD camera with picosecond resolution.
    • Acquired time-delayed fluorescence images from cyanine 3-marked DNA microarrays.
    • Measured fluorescence lifetimes of the marker and background.

    Related Experiment Videos

  • Calculated marker amplitude maps from time-delayed images.

    • Main Results:

    • Successfully identified hybridized spots invisible with continuous-wave detection.
    • Demonstrated the effectiveness of time-resolved fluorescence for enhanced signal detection.
    • The amplitude map provided a clearer signal-to-noise ratio.

    Conclusions:

    • Time-resolved fluorescence measurements offer a significant advancement in DNA microarray reading.
    • This novel technique improves the sensitivity and accuracy of genetic analysis.
    • The method enables the detection of previously undetectable hybridization events.