Stepwise functionalization of ribonucleopeptides: optimization of the response of fluorescent ribonucleopeptide
Tetsuya Hasegawa1, Masaki Hagihara, Masatora Fukuda
1Institute of Advanced Energy, Kyoto University, Uji, Kyoto, Japan.
Nucleosides, Nucleotides & Nucleic Acids
|December 11, 2007
Abstract:
A stable complex of a peptide and RNA, ribonucleopeptide (RNP), provides a new framework to construct a macromolecular receptor for small molecules. The RNP receptor functionalized by a fluorophore-labeled Rev peptide exerts an optical signal associated with the ligand binding events. Replacing the Rev peptide of the ATP-binding RNP with a fluorophore-modified Rev peptide affords a fluorescent ATP sensor.
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