Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 9, 2026

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

Optical tweezers as a probe for oligodeoxyribonucleotide structuration.

Nicola Borbone1, Giorgia Oliviero, Jussara Amato

  • 1Dipartimento di Chimica delle Sostanze Naturali, Università di Napoli Federico II, Faculty of Pharmacy, via Domenico Montesano 49, Naples 80131, Italy. borbone@unina.it

Nucleosides, Nucleotides & Nucleic Acids
|December 11, 2007
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Explainable deep-learning detection of microplastic fibers via polarization-resolved holographic microscopy.

Optics express·2026
Same author

Epigenetic mutations: Effects on the stability of G-quadruplex structures in the c-KIT promoter.

International journal of biological macromolecules·2026
Same author

Advances in Digital Holography, 3-D Imaging, and Optical Information Processing: introduction.

Biomedical optics express·2026
Same author

Ligands reshape the compactness, stability, and topology of telomeric G-quadruplex dimers.

Nucleic acids research·2026
Same author

Advances in digital holography, 3-D imaging, and optical information processing: introduction.

Applied optics·2026
Same author

Advances in digital holography, 3-D imaging, and optical information processing: introduction.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026

Optical tweezers can monitor DNA structure changes in solution by detecting viscosity shifts during folding and unfolding. This technique shows promise for diagnosing oligodeoxyribonucleotide structural behavior.

Area of Science:

  • Biophysics
  • Molecular Biology
  • Nanotechnology

Background:

  • Oligodeoxyribonucleotides (ODNs) exhibit complex structural behaviors in solution, crucial for their function.
  • Monitoring these structural dynamics is essential for understanding ODN applications and developing diagnostic tools.

Purpose of the Study:

  • To evaluate the suitability of optical tweezers (OT) as a diagnostic method for assessing ODN structural changes.
  • To investigate the correlation between ODN folding-unfolding processes and changes in solution viscosity.

Main Methods:

  • Utilized optical tweezers (OT) to probe an oligodeoxyribonucleotide (ODN) solution.
  • Focused on the quadruplex structure formed by the TGGGGT ODN sequence.
  • Monitored changes in the medium's viscosity as an indicator of ODN structural transitions.

More Related Videos

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
12:26

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies
06:53

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

Related Experiment Videos

Last Updated: Jul 9, 2026

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
12:26

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies
06:53

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

Main Results:

  • Demonstrated that optical tweezers can detect viscosity variations in solution.
  • Observed that these viscosity changes are associated with the folding and unfolding of the TGGGGT ODN.
  • Confirmed the potential of OT to track ODN structuration processes.

Conclusions:

  • Optical tweezers are a viable tool for monitoring oligodeoxyribonucleotide structural behavior in solution.
  • The technique allows for the detection of ODN folding-unfolding dynamics via viscosity measurements.
  • OT offers a novel approach for the diagnostic assessment of ODN structural states.