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Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
Published on: May 30, 2025
Survivin mRNA antagonists using locked nucleic acid, potential for molecular cancer therapy
Niels Fisker1, Majken Westergaard, Henrik Frydenlund Hansen
1Santaris Pharma A/S, Boege Allé 3, DK-2970 Hoersholm, Denmark. NFN@santaris.com
Abstract:
We have investigated the effects of different locked nucleic acid modified antisense mRNA antagonists against Survivin in a prostate cancer model. These mRNA antagonists were found to be potent inhibitors of Survivin expression at low nanomolar concentrations. Additionally there was a pronounced synergistic effect when combining the mRNA antagonists against Survivin with the chemotherapeutic Taxol. This effect was demonstrated at concentrations of antagonists far lower than any previously demonstrated, indicating the high potential of locked nucleic acid for therapeutic use. Further characterisations in vivo are ongoing.
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