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Updated: Jul 9, 2026

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
Published on: November 1, 2012
Mismatch discrimination of base-modified nucleic acids and their constituents: non-Watson-Crick base pairing induced
Frank Seela1, Xiaohua Peng, Kuiying Xu
1Laboratorium für Organische und Bioorganische Chemie, Universität Osnabrück, Barbarastrasse 7, 49069 Osnabrück, Germany. Frank.Seela@uni-osnabruck.de
Abstract:
The effect of tautomerism on the mismatch discrimination was studied on 7-deazapurine and 8-aza-7-deazapurine analogues of isoguanosine. 7-Halogenated 7-deaza-2'-deoxyisoguanosines show better base pair discrimination than 2'-deoxyisoguanosine due to the more favored keto tautomer formation. 8-Aza-7-deazaisoguanosine and its 7-halogeno derivatives also show higher keto tautomer population than that of isoguanosine, but the 7-halogens do not bias the tautomeric equilibrium significantly as it is observed for the 7-deaza-2'-deoxyisoguanosine derivatives.
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