Related Experiment Video
Updated: Jul 9, 2026

05:33
Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes
Published on: July 5, 2024
Novel fluorophores for labeling of nucleosides and oligonucleotides
Shipra Singh1, Ramendra K Singh
1Nucleic Acids Research Laboratory, Department of Chemistry, University of Allahabad, Allahabad 211002, India.
Nucleosides, Nucleotides & Nucleic Acids
|December 11, 2007
Abstract:
Five novel fluorophores have been synthesized and their comparative fluorescence has been studied in different organic solvents and aqueous solutions of inorganic ions. Out of these, two highly sensitive fluorophores have been used to label phosphoramidites and oligodeoxyribonucleotides. The fluorescently labelled amidites and oligodeoxyribonucleotides showed good fluorescence signals.
Related Concept Videos
Labeling DNA Probes
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Photoluminescence: Applications
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

