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Updated: Jul 9, 2026

DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes
Published on: March 31, 2019
Thiazole orange: a useful probe for fluorescence sensing of G-quadruplex-ligand interactions
D Monchaud1, C Allain, M-P Teulade-Fichou
1Section Richerche, Institut Curie, CNRS UMR 176, Centre Universitaire Paris XI, 91405 Orsay, France.
Abstract:
Fluorimetric titrations were performed to gain insight into parameters that govern the association of thiazole orange (TO) and G-quadruplex-DNA (G4-DNA). Use of loop-containing and loop-lacking quadruplexes evidenced the critical influence of the loops on the stoichiometry of the association and on the fluorescence exaltation of TO. We subsequently tried to benefit from this sensitivity to evaluate the influence of G4-DNA cationic environment on ligand binding via a recently reported G4-FID assay.
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