Synthesis of DNA-oligonucleotides damaged by arylamine-modified 2'-deoxyguanosine
1Department of Chemistry, Organic Chemistry, Faculty of Science, University of Hamburg, Hamburg, Germany. boege@chemie.uni-hamburg.de
Nucleosides, Nucleotides & Nucleic Acids
|December 11, 2007
Abstract:
C8-Arylamine-dG adducts bearing a labile N-formamidine group at the exocyclic amino function were converted into their corresponding 5'-O-DMTr-3'-O-phosphoramidite-C8-arylamine-dG derivatives. These compounds were used for the automated synthesis of site-specifically modified oligonucleotides. These oligonucleotides were characterized by ESI-MS and enzymatic digestion and studied for their CD properties and Tm values.
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