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Updated: Jul 9, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
[Identification of closely related bacteria via phylogenetic methods]
Qing-yi Cao1, Xiao-li Hou, Hao Wu
1Institute of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Objective:
To differentiate closely related pathogenic bacteria via phylogenetic method on the basis of gyrB gene sequences.
Methods:
GyrB sequences of 19 strains of E.coli, 13 Shigella spp. 2 Aeromonas caviae, 2 Aeromonas hydrophilia,1 Aeromonas veronii were determined and combined with sequences retrieved from public databases to construct phylogenetic trees. For each sequence tested, the identification deduced from the clustering relation of sequences was compared with the phenetic identification.
Results:
All the tested sequences, except those of Shigella boydii and Shigella dysenteriae, were corresponded with the 5 closest sequences on the tree at the species level. While the BLAST queries returned some other bacteria organisms or undetermined entries.
Conclusion:
Phylogenetics displays good discriminative power in bacterial sequences differentiation.
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