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Ischemic Tissue Injury in the Dorsal Skinfold Chamber of the Mouse: A Skin Flap Model to Investigate Acute Persistent Ischemia
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[Spontaneous response and mechanism to ischemia in diabetic mouse]
Yong-jun Li1, Qing Lin, Chang-wei Liu
1Department of Vascular Surgery, Peking Union Medical College Hospital, and Chinese Academy of Medical Sciences, Beijing 100730, China. yongj_93@hotmail.com
Objective:
To investigate the spontaneous response and its mechanism of type 2 diabetes mouse to surgical induced hind limb ischemia.
Methods:
Forty mice were randomly divided into two groups, one was fed with normal chow, and another was fed with high fat diet. Twelve weeks later, mice were surgical induced to hind limb ischemia. Blood flow was monitored. Four weeks later, mice were sacrificed and muscle tissues were harvested for histological detection, Western Blot, ELISA and RT-PCR examination respectively.
Results:
Spontaneous restoration of blood flow was significantly slow down in type 2 diabetic mice with lower level of vascular density in ischemic muscles. VEGF signaling pathway was impaired although the VEGF levels were increased in diabetes.
Conclusions:
The spontaneous response to ischemia was attenuated with an abnormal reaction of VEGF signaling pathway in the setting of type 2 diabetes mellitus.
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