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[Experimental data on the virological study of pasteurized milk]
Abstract:
Virological analyses of sterile milk per se enabled the poliovirus to be disclosed with a concentration of 30 infection units in 1 ml. To determine such doses of the viruses in pasteurized milk the viruses have to be concentrated, for prior to cytopathogenic manifestations subsequent to these viral doses there occurs bacterial germination. In achieving poliovirus concentration in pasteurized milk are suitable polyethyleneglycol, molecular weight 15 000, and polyvinylpyrolidon, molecular weight 10 000, in a 10% concentration. Dilution of milk with Hanks' solution in the ratio of 1:4 and 1:9 improves the process of the poliovirus sorption-eluation with the polymer, enabling it to detect 3 BOU of the virus in 1 ml of milk. The AB-17-8 anionite and Ky-21-8 cationite are capable of adequately sorbing the poliovirus in pasteurized milk, but it is only from the cationite, and then only in a small amount, that eluation of the virus can be accomplished.
Insights
Concentrating poliovirus in pasteurized milk is crucial for detection. Polyethylene glycol and polyvinylpyrrolidone effectively concentrate poliovirus, enabling detection of 3 infection units per ml.
Area of Science:
- Food Safety
- Virology
- Microbiology
Context:
- Detecting poliovirus in pasteurized milk presents challenges due to bacterial interference.
- Accurate virological analysis requires effective concentration methods for low viral loads.
Purpose:
- To develop and evaluate methods for concentrating poliovirus in pasteurized milk.
- To improve the sensitivity of poliovirus detection in milk samples.
Summary:
- Virological analysis of sterile milk can detect poliovirus at 30 infection units/ml.
- Concentration of poliovirus in pasteurized milk is necessary before cytopathogenic effect observation due to bacterial germination.
- Polyethylene glycol (PEG) and polyvinylpyrrolidone (PVP) at 10% are effective for poliovirus concentration.
- Dilution of milk with Hanks' solution (1:4, 1:9) enhances poliovirus sorption-elution with polymers, allowing detection of 3 BOU/ml.
- Anionite (AB-17-8) and cationite (Ky-21-8) can sorb poliovirus, with limited elution possible from cationite.
Impact:
- Establishes effective methods for poliovirus concentration in pasteurized milk.
- Enhances the ability to detect low levels of poliovirus contamination in milk.
- Contributes to improved food safety surveillance and public health protection against poliovirus.