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Updated: Jul 9, 2026

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
Published on: August 16, 2018
Inheritance and expression of transgenes through anther culture of transgenic hot pepper
Young Soon Kim1, Yong In Kuk, Kyung-Moon Kim
1Kumho Life and Environmental Science Laboratory & Agricultural Plant Stress Research Center, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea.
Abstract:
Anther cultures have been developed from transgenic donor peppers carrying HPT/J1-1. Eight out of sixteen plants produced from an anther culture set pepper fruits. Southern blot analysis of donors revealed two independent plants with a single copy of the integrated transgene. PCR and RT-PCR results showed the inheritance of HPT/J1-1 and expression of J1-1 in A1. All A1 progeny derived from transgenic anthers had resistance to hygromycin. They grew normally and showed similar phenotypes to the wild-type. Therefore, the use of an anther culture system coupled with genetic transformation in breeding programs will greatly facilitate the genetic improvement of pepper plants.
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