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Updated: Jul 9, 2026
A High-throughput Compatible Assay to Evaluate Drug Efficacy against Macrophage Passaged Mycobacterium tuberculosis
Published on: March 24, 2017
[Rapid testing on drug susceptibility of Mycobacterium tuberculosis with nitrate reducrase assay]
Zhong-Quan Liu1, Chuan-You Li, Xiao-You Chen
1Department of Bacteriology and Immunology, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, China.
Objective:
To establish a rapid method for testing drug sussceptibility on Mycobacterium tuberculosis.
Methods:
Taking absolute Concentration method for drug susceptibility testing of M. tuberculosis as the "gold standard", we examined the drug-resistant of M. tuberculosis strain with nitrate reducrase assay (NRA) and the drug-resistant of M. tuberculosis germ in sputum with NRA.
Results:
NRA and absolute concentration method was basically comparable with NRA susceptibility as 96.5% and the specificity was 100%, When comparing with traditional absolute concentration method, NRA could shorten the time about 3 weeks. Using NRA to test the drug-resistant of M. tuberculosis germ in sputum, its susceptibility was more than 66.7% and specificity was 100%, within 10-20 days.
Conclusion:
NRA could be used as a rapid drug susceptibility testing on M. tuberculosis.