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Updated: Jul 9, 2026
Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
Effect and mechanism of beta-L-D4A on DNA polymerase alpha
Yan Li1, Ju-Sheng Lin, Ying-Hui Zhang
1Institute of Liver Diseases, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Street, Wuhan 430030, Hubei Province, China.
Aim:
To investigate the safety of beta-L-D4A on DNA polymerase alpha.
Methods:
Ion exchange chromatography was used to separate DNA polymerase alpha from crude extract of human Hela cells. Detailed kinetic parameters were determined for beta-L-D4A against DNA polymerase alpha.
Results:
DNA polymerase alpha was purified with 4% yield and 31000 units/mg specific activity. The Michaelis constant (Km = 3.22 micromol/L), 50% inhibition concentration (IC50 = 178.49 micromol/L) and inhibition constant (Ki = 126 micromol/L) of beta-L-D4A were determined by kinetic analysis.
Conclusion:
beta-L-D4A is a more safe nucleoside for hepatitis B virus infection with a lower host toxicity.
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