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Related Concept Videos

Meiosis I01:49

Meiosis I

Meiosis is a carefully orchestrated set of cell divisions, the goal of which—in humans—is to produce haploid sperm or eggs, each containing half the number of chromosomes present in somatic cells elsewhere in the body. Meiosis I is the first such division, and involves several key steps, among them: condensation of replicated chromosomes in diploid cells; the pairing of homologous chromosomes and their exchange of information; and finally, the separation of homologous chromosomes by a...
Meiosis II01:57

Meiosis II

Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 chromosomes—one from each homologous initial pair. Importantly, each chromosome in these cells is composed of two joined copies, and when these cells enter meiosis II, the goal is to separate such sister chromatids using the same microtubule-based network employed in other division processes. The result of meiosis II is two haploid cells, each containing...
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
Meiosis I03:09

Meiosis I

Meiosis is the division of a diploid cell into haploid cells forming sperm and eggs in animals through differentiation. Meiosis I is the first stage of meiosis, where the genetic recombination of homologous chromosomes and the reduction of the ploidy level by half occurs.
Prophase I is the most extended and complex step of meiosis I characterized by synapsis, chromosome pairing, and recombination of the homologous chromosomes. This process is facilitated by a proteinaceous structure called the...
Meiosis II02:02

Meiosis II

Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
Meiosis I03:09

Meiosis I

Meiosis is the division of a diploid cell into haploid cells forming sperm and eggs in animals through differentiation. Meiosis I is the first stage of meiosis, where the genetic recombination of homologous chromosomes and the reduction of the ploidy level by half occurs.
Prophase I is the most extended and complex step of meiosis I characterized by synapsis, chromosome pairing, and recombination of the homologous chromosomes. This process is facilitated by a proteinaceous structure called the...

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Related Experiment Video

Updated: Jun 26, 2026

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye
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Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye

Published on: January 14, 2021

Novel roles for selected genes in meiotic DNA processing.

Philip W Jordan1, Franz Klein, David R F Leach

  • 1Institute of Cell Biology, University of Edinburgh, Edinburgh, United Kingdom.

Plos Genetics
|December 12, 2007
PubMed
Summary

Researchers identified novel genes in yeast meiosis using computational analysis and experimental testing. This study highlights Def1, Soh1/Med31, Bre5, and Rmr1/Ygl250w

Area of Science:

  • Molecular Biology
  • Genetics
  • Yeast Biology

Background:

  • High-throughput studies in Saccharomyces cerevisiae yield vast data.
  • Experimental validation is crucial for interpreting these large datasets.
  • Identifying genes with specific roles in meiosis requires targeted approaches.

Purpose of the Study:

  • To develop an in-silico screening method for identifying genes involved in meiotic DNA processing.
  • To experimentally validate the meiotic roles of selected, minimally characterized genes.
  • To uncover novel genes participating in DNA replication, recombination, and chromosome segregation during meiosis.

Main Methods:

  • In-silico screening of existing Saccharomyces cerevisiae datasets (interaction, expression, localization, phenotype).

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Preparation of Meiotic Chromosome Spreads from Mouse Oocytes for Assessment of Synapsis and Recombination
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Preparation of Meiotic Chromosome Spreads from Mouse Oocytes for Assessment of Synapsis and Recombination

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Last Updated: Jun 26, 2026

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Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
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  • Construction and phenotypic analysis of 81 deletion mutants.
  • Focus on genes with potential roles in meiotic DNA processing.

    • Main Results:

    • Eleven (13.6%) genes were identified with novel roles in meiotic DNA processes.
    • Def1 is essential for homologous synapsis and crossover recombination during meiosis.
    • Soh1/Med31, Bre5, and Rmr1/Ygl250w are required for normal gene conversion events.

    Conclusions:

    • Existing datasets can be mined to identify gene sets enriched for specific biological roles.
    • Experimental evaluation is effective in confirming predicted gene functions.
    • This study expands the understanding of genetic regulation in yeast meiotic DNA processing.