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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies
08:00

Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

Published on: November 4, 2021

Plant peptides and peptidomics.

Naser Farrokhi1, Julian P Whitelegge, Judy A Brusslan

  • 1National Institute of Genetic Engineering and Biotechnology, Pajoohesh Blvd., Tehran-Karaj Highway, 17th Km., Tehran, Iran. nfarrokh@nigeb.ac.ir

Plant Biotechnology Journal
|December 12, 2007
PubMed
Summary

Peptidomics, a proteomics method, rapidly identifies plant peptides, including those involved in signaling and defense. This review explores known plant peptides and peptidomics for discovering new ones.

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Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus
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Last Updated: Jul 9, 2026

Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies
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Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

Published on: November 4, 2021

Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus
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Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus

Published on: May 31, 2022

Area of Science:

  • Plant biology
  • Proteomics
  • Biochemistry

Background:

  • Extracellular plant peptides are crucial for signaling and defense.
  • Intracellular peptides have physiological roles or result from proteolysis.
  • Traditional methods for plant peptide identification are time-consuming and often impractical.

Purpose of the Study:

  • To review known plant peptides and their functions.
  • To introduce peptidomics as a novel methodology for plant peptide detection.
  • To highlight the advantages of peptidomics over traditional methods.

Main Methods:

  • Peptidomics, a proteomics technique, was employed.
  • This method allows for the detection of minute and transient peptide amounts.
  • Identification of post-translational modifications is a key feature.

Main Results:

  • Peptidomics offers a fast and efficient approach to peptide analysis.
  • The methodology is suitable for identifying novel plant peptides.
  • It complements existing biochemical and genetic studies.

Conclusions:

  • Peptidomics is a powerful tool for expanding the understanding of plant peptide diversity.
  • This technique facilitates the discovery of previously uncharacterized peptides.
  • The application of peptidomics promises significant advancements in plant science research.