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An In Vitro Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model

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Assessing skin prick tests reliability in ECRHS-I.

P-J Bousquet¹, L Chatzi, D Jarvis

¹Respiratory Epidemiology and Public Health, Imperial College, London, UK.

|December 12, 2007

Summary

A skin prick test cut-off of over 0 mm is best for identifying individuals with allergen-specific immunoglobulin E (IgE) in epidemiological studies. This finding aids in standardizing allergy assessment methods.

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Published on: August 23, 2015

Area of Science:

Allergy and Immunology
Epidemiology
Respiratory Medicine

Background:

Atopy, defined as immunoglobulin E (IgE) high-responder status, is clinically assessed via serum IgE or skin prick tests (SPT).
Epidemiological studies vary in defining positive SPT reactions, necessitating standardization.
The study aimed to determine the optimal SPT cut-off for allergy assessment.

Purpose of the Study:

To identify the most effective cut-off level for skin prick tests in epidemiological studies.
To compare the diagnostic performance of different SPT cut-offs against serum allergen-specific IgE levels.

Main Methods:

Utilized data from the European Community Respiratory Health Survey (ECHRS I).
Assessed the association between serum allergen-specific IgE and SPT results for Dermatophagoides pteronyssinus (Der p), cat, timothy grass, and Cladosporium.
Evaluated various SPT cut-off levels (e.g., >5 mm, >0 mm).

Main Results:

SPT positive rates varied significantly by allergen and cut-off level.
A cut-off of >0 mm for SPT demonstrated the highest accuracy (Youden Index >0.81) in identifying subjects with detectable specific IgE for Der p, cat, and timothy grass.
The correlation between serum IgE and SPT for Cladosporium was weak (Youden Index <0.56).

Conclusions:

A single method can be used for assessing allergenic sensitivity in epidemiological research.
An SPT cut-off of >0 mm is recommended for identifying individuals with allergen-specific IgE, enhancing consistency in allergy studies.