Related Experiment Video
Updated: Jul 9, 2026
Fabrication of Tongue Extracellular Matrix and Reconstitution of Tongue Squamous Cell Carcinoma In Vitro
Published on: June 20, 2018
[Proteomics study of the serum biomarkers in tongue cancer]
Xin Huang1, Fei-Wu Kang, Long-Jiang Li
1Department of Oral and Maxillofacial Surgery, Faculty of Stomatology, Capital University of Medical Sciences, Beijing 100050, China. huangxin_291@hotmail.com
Objective:
To search for specific serum biomarkers associated with tongue cancer by means of the serum proteomics technology.
Methods:
The tongue cancer cells of human tongue cancer cell line Tca8113 were subcutaneously inoculated into nude mice, while control nude mice were injected with phosphate-buffered saline. Serums from these two group of mice were collected for proteomic analysis using two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS).
Results:
Comparing the serum 2-DE maps from the tumor-bearing mice with those produced from control mice, we found that squamous cell carcinoma antigen 1 was over expressed only in the serum of tumor-bearing mice.
Conclusions:
The squamous cell carcinoma antigen 1 may be of great potential as the biomarker of tongue cancer and as the potential therapeutic target for gene therapy.
Related Concept Videos
01:30Serum Laboratory Studies, Stool Test, Breath Test
01:26Assessment of the Mouth
Mouth Inspection
The inspection begins with visually examining the mouth for symmetry, color, and size.