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[Construction of anti-lung tumor gene differentially expressed bank of wild mouse]
Feng-shan Wang1, Ying-tang Gao, Jiang-shan Yang
1Tianjin Centers for Disease Control and Prevention, Tianjin 300011, China.
Objective:
To construct the anti-lung tumor gene differentially expressed bank of wild mouse and to explore the mechanisms of the TW wild mouse suppressing the occurring of lung tumor.
Methods:
Using suppression subtractive hybridization (SSH) technique, the differentially expressed genes between TAF1 mouse and A/wy mouse were selected out and the subtracted cDNA bank was constructed. 166 clones were performed DNA sequencing and then were assayed by blast programme.
Results:
Among the blast results of 166 differentially expressed clones, 87 known genes (mRNA or cDNA) were in homology with 134 clones and were divided into 7 classifications according to the biological role.14 DNA fragments were in homology with 32 clones, in which 20 clones were in homology with 9 mouse DNA sequences, 2 clones were in homology with one bacterial gene sequences and 3 clones were clone vector.
Conclusion:
With SSH technique, the anti-lung tumor gene differentially expressed bank of wild mouse are successfully constructed.
