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Updated: Jul 9, 2026
Extraction of Plant-based Capsules for Microencapsulation Applications
Published on: November 9, 2016
Purification of elastase-like chymotrypsin from cardamom shoot and Capsule borer [corrected]
A Josephrajkumar1, R Chakrabarty, G Thomas
1Cardamom Research Station, Kerala Agricultural University, Pampadumpara 685 556, Dist. Idukki, India. entojoe2003@yahoo.co.in
Abstract:
An elastase-like chymotrypsin was purified by aprotinin-agarose affinity chromatography from the midgut extract of cardamom shoot and capsule borer, Conogethes punctiferalis. The purified enzyme had a Vmax of 687.6 +/- 22.1 nmole pNA released/min/mg protein, Km of 0.168 +/- 0.012 mM with SAAPLpNA as substrate and gave a single band on SDS-PAGE with a molecular mass of 72.1 kDa. Casein zymogram revealed one clear zone of proteolytic activity, which corresponded to the band obtained with SDS-PAGE indicating that this could be a single-polypeptide enzyme.
