Related Experiment Video
Updated: Jul 9, 2026

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
Published on: May 21, 2015
[Distribution of five periodontal pathogens in subgingival plaque in chronic periodontitis]
1Department of Oral Medicine, Stomatological College of Chongqing University, Chongqing, China.
Objective:
To investigate the distribution of H. actinomycetemcomitans, P. gingivalis, P. intermedia, T. forsythensis and T. denticola in the subgingival plaque in the patients with chronic periodontitis.
Methods:
27 patients with chronic periodontitis were included. Two of the deepest pockets of each patient were selected as the study sites. Semi-quantification of subgingival microorganism samples was analyzed by multiplex polymerase chain reaction and reverse hybridization assay.
Results:
P. gingivalis, P. intermedia, T. forsythensis and T. denticola were detected in a high proportion of examined sites(corresponding values were 98.15%, 92.59%, 100% and 98.15%), however, the proportion of H. actinomycetemcomitans was low (20.37%). The levels of P. gingivalis and T. forsythensis were higher than the other three microorganisms with statistical significance.
Conclusion:
The simultaneous infection of P. gingivalis, T. forsythensis, P. intermedia and T. denticola is found in the patients with chronic periodontitis, in which the levels of P. gingivalis and T. forsythensis are higher than the other two microorganisms.
Related Concept Videos
The Oral Microbiota
Bacterial Phylum Spirochaetes
Bacterial Phylum Proteobacteria
Bacterial Phylum Bacteroidota
Bacterial Phylum Planctomycetes
Determinants of Bacterial Pathogenicity and Virulence
