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Related Experiment Video

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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MudPIT: multidimensional protein identification technology.

Claire M Delahunty1, John R Yates

  • 1Department of Physiological Chemistry, The Scripps Research Institute, La Jolla, CA, USA.

Biotechniques
|December 13, 2007
PubMed
Summary
This summary is machine-generated.

Large-scale biology leverages whole genome sequencing for analyzing gene and protein expression. Advanced mass spectrometry and bioinformatics rapidly identify proteins from complex peptide mixtures.

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Area of Science:

  • Genomics
  • Proteomics
  • Bioinformatics

Background:

  • Large-scale biology research is driven by advancements in whole genome sequencing.
  • Genomic data enables large-scale analyses of messenger RNA (mRNA) and protein expression.

Purpose of the Study:

  • To describe the emergence and methodology of large-scale protein identification.
  • To highlight the role of mass spectrometry and bioinformatics in analyzing complex biological samples.

Main Methods:

  • Proteolytic digestion of proteins into peptides.
  • Peptide fragmentation using collision-activated dissociation (CAD) in tandem mass spectrometers.
  • Computer algorithms for matching tandem mass spectra to sequence databases.

Main Results:

  • Rapid identification of amino acid sequences and proteins from complex mixtures.
  • Development of methods for analyzing complex peptide mixtures using mass spectrometry.

Conclusions:

  • Whole genome sequencing has facilitated large-scale biological analyses.
  • Mass spectrometry coupled with bioinformatics is crucial for protein identification and analysis in large-scale biology.