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A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
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Published on: June 20, 2020

Two-dimensional angular optical scattering patterns as droplets evolve into clusters.

S Holler, M Surbek, R K Chang

    Optics Letters
    |December 13, 2007
    PubMed
    Summary
    This summary is machine-generated.

    Acoustic levitation reveals how inclusions in water droplets create a unique speckle pattern in optical scattering. This pattern emerges as the droplet dries, indicating a significant change in its optical properties.

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    Published on: December 8, 2016

    Area of Science:

    • Fluid dynamics
    • Optical physics
    • Materials science

    Background:

    • Acoustically levitated droplets offer a controlled environment for studying phase transitions.
    • Optical scattering patterns, like Mie scattering, are sensitive to droplet morphology.
    • The effect of internal inclusions on droplet scattering is not fully understood.

    Purpose of the Study:

    • To investigate the changes in two-dimensional angular optical scattering (TAOS) patterns of acoustically levitated water droplets containing inclusions as they transition to dry aggregates.
    • To determine the influence of inclusion emergence and volume fraction on TAOS patterns.
    • To characterize the optical signature of droplet evaporation and aggregation.

    Main Methods:

    • Acoustic levitation was used to suspend water droplets containing polystyrene latex spheres.
    • Two-dimensional angular optical scattering (TAOS) patterns were measured during droplet evaporation.
    • The evolution of scattering patterns was correlated with the droplet's physical state and inclusion distribution.

    Main Results:

    • The emergence of inclusions near the droplet surface induced a transition from continuous Mie scattering contours to a distinct speckle pattern in the TAOS.
    • This speckle pattern persisted throughout the evaporation process, marking the transition to a dry aggregate.
    • The onset of the speckle pattern was observed at an inclusion volume fraction of approximately 8%.

    Conclusions:

    • The TAOS pattern is highly sensitive to the presence and distribution of inclusions within evaporating droplets.
    • The transition to a speckle pattern serves as a reliable indicator of the formation of a dry aggregate from a droplet with inclusions.
    • This phenomenon provides a novel optical method for monitoring droplet drying and aggregation processes.