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Updated: Jul 9, 2026
High-resolution Tandem Mass Spectrometry for Studying Chemical Constituents of Gynura bicolor DC
Published on: February 2, 2024
[Studies on the HPLC fingerprint spectrum of flavonoids in Gynostemma pentaphyllum]
Jin-qing Lu1, Li Chen, Bo Xiao
1Hubei College of Traditional Chinese Medicine, Wuhan 430061, China. ljq59169@sohu.com
Objective:
To establish the HPLC fingerprint spectrum of flavonoids from Gynostemma pentaphyllum.
Methods:
The HPLC conditions with TURNER Kromasil C18 column (250 x 4. 6 mm, 5 microm) was used, the mobile phase consisted of 0.4% phosphoric acid-methanol-acetonitrile with gradient elution at flow rate of 0.5 ml/min and the detection wavelength was set at 360nm.
Results:
Ten common characteristic peaks were taken as fingerprint peaks the presision and the retest are in accordance with the requirement.
Conclusion:
The method is stable, reliable, precision and provides a scientific basis for the quality standard of Gynostemma pentaphyllum.
